Solutions & Technology

 

Glycoanalysis

Accurate analysis of protein glycosylation is crucial for a range of applications, including :  process development & manufacturing of biopharmaceuticals; life science & pharma research; glycodiagnostic & biomarker tools; stem cells; and food applications.

Procognia's advanced, validated, proprietary technology for high throughput glycoanalysis on lectins array is the fastest and most effective solution, applicable for the above applications.

Procognia's glycoanalysis solution is delivered in three products developed with the technical know how and experience of the company:

1. GlycoScopeTM

GlycoScopeTM is an integrated technological platform for the analysis of recombinant monoclonal antibodies and complex biopharmaceutical glycoproteins.

GlycoScopeTM enables analysis of dozens of samples concurrently for same-day resultsrofilethe worldwide exclusive license for the use of this technology for biosimilar applications.y processes, for an e, and analysis can be performed on cell lines supernatants and bioreactor crude harvest samples with minimal sample requirements and enrichment. Results are rapid - up to 6 hours - and automatic data analysis is complete within 1 to 2 minutes. Glycoscope TM is user-friendly, requiring basic laboratory skills for easy operation.

 

GlycoScopeTMAnalysis Provides:

Quantitative glycan distribution list - the relative distribution of glycan types and epitopes. Quantitative results are calculated from the fingerprint signals using proprietary algorithms.

Fingerprint - a graphic representation of the binding signals. Lectins are grouped according to their binding specificity. The fingerprint is highly sensitive to changes in the glycosylation pattern of the sample

 

 

   

    2. Qproteome GlycoArray kitsTM

The Qproteome GlycoArrayTM is an off-the-shelf kit for  glycoanalysis of purified proteins. Launched in 2006, this innovative product provides a fast and simple tool for 4-level quantification glycoanalysis, with the capability of analyzing up to 12 samples concurrently. Designed for low resolution glycoanalysis for a large range of samples, the product features manual processing, stand-alone software and can be used with the customer's scanner.

Qproteome GlycoArrayTM kits are distributed by QIAGEN (Cat. No. 37702).

To order, visit http://www1.qiagen.com/products/protein/proteomics/Qproteome/QproteomeGlycoArrayKit.aspx

 

3. Qglycome Cell Glycoprofiling kitsTM

  The Qglycome Cell GlycoprofilingTM kit is a user-friendly, off-the-shel 

  method for comparing the glycosylation pattern of membrane protein extracts from mammalian cells. This product provides a simple and rapid tool   for the comparative analysis of two samples, the reference sample and     the test sample. Examples include cells before and after differentiation,  and cells in comparison to various biological, chemical and/or physical stimuli.

 

Biosimilar Preclinical Development Package
The development of biosimilar drugs is a complex, multi-faceted process that presents many obstacles. Procognia offers a comprehensive solution package encompassing the entire pre-clinical development process, to assist you on a highly efficient path to achieving regulatory approval.

Procognia's state-of-the-art package comprises a synergy of validated proprietary technologies and tools to take you from clone generation, through to purified bulk product for pre-clinical studies. Every element of the Procognia package is conducted by specialist experts utilizing the highest caliber technologies and techniques. The result is a unified management of complementary processes, for a highly effective and low risk profile.

 

High Expression Clone Selection
Procognia holds the exclusive rights for a proprietary technology for high expression clone generation, developed by a technology company exploiting mRNA targeting and other technologies to increase protein synthesis in mammalian cells. This high expression technology provides yield enhancement compatible with most existing technological platforms and has been validated by leading biotech companies on their optimized expression platforms.

      The high expression technology with the wide      screening for the best producer clone in combination with glycosylation pattern analysis increases the probability of identifying the most suitable clone for both productivity and biosimilarity at a very early stage of development. 

 

 Procognia High Expression Advantages

Highly effective mRNA targeting elements
Increased productivity
Reduced COG
Fast time-to-market
High return on investment